Antibiotic Detection Kit

One of the most frequently used drugs in the treatment of various infectious diseases in humans and animals are antibiotics. Excessive use of this kind of drug, in livestock results in antibiotic residues in their raw milk which cause health problems in human communities. Today, by developing new methods in animal husbandry, using antibiotics to control mastitis, local diseases, etc. has become common. Concerns about veterinary drug residues in food have increased largely that Food safety organizations emphasize the necessity of food being free of such residues. Currently, all antibiotic test kits for milk are imported and have a relatively high cost. This high cost has led dairy companies to use cheaper brands with large performance errors or to use these kits just to control the raw milk consumed in their fermented products. The regular antibiotic rapid test kit based on nanoparticles specified for the intended antibiotic. When they come into contact with a certain amount of raw milk (usually, temperature acts as a catalyst) will absorb antibiotics if present.
By inserting a paper strip into the sample, the solution moves to the top of the strip, and if the nanoparticles are released, they are absorbed at specific sites, forming a red line, which means that the antibiotic is less than the allowable limit. If the red line does not form, means that the antibiotic is positive. To detect drug residues in food industry various quantitative and qualitative methods such as spectrophotometry, chromatography, electrophoresis, microbiology and immunochemistry are used, but these methods cannot be used in the dairy industry due to high cost and long time they take. In addition to milk antibiotic rapid test kits, there are several other methods for detecting antibiotics in raw milk, including:

 

- To use an HPLC machine is expensive and time consuming that requires a skilled operator.
- To use ELISA method which is expensive and time consuming that requires a skilled operator.
- Clot testing which has fair price but it’s time consuming and the diagnosis accuracy is very low.
- Coupon kit, which is time-consuming.
Generally, In the dairy industry, milk is exposed to a lot of chemical contaminants. These contaminants enter milk through various means such as livestock treatment, feed, milking environment and production process. Therefore, it is essential to minimize the risk of milk contamination with careful control. Chemical contaminants in milk include: antibiotics, hormones, disinfectants, nitrites, nitrates and nitrosamines, insecticides, fungal toxins, toxic metals, dioxins, etc. Antibiotics are products of the secondary metabolism of microorganisms that have the ability to kill bacteria and prevent them from growing. Antibiotics are used to control systemic and local diseases in livestock. The most commonly use of antibiotic is mastitis control. Fat-soluble antibiotics stay in the breast longer than water-soluble antibiotics. Because ranchers use antibiotics to treat microbial diseases in their herds, these drugs enter the animal's body, including their milk. After entering the body, Antibiotic needs a certain amount of time to decompose and excrete by animal. According to the following reasons, it’s not allowed to use raw milk which contains antibiotic residues in the dairy industry. (It should be noted that antibiotics are resistant to pasteurization and sterilization process temperature)

 

 

1- This milk cannot be used in the production of fermented products, because antibiotics prevent the growth of beneficial microorganisms used in these products, or disrupt their growth, so the product produced from this milk will not be of good quality.
2- Consumption of dairy products containing antibiotic residues greater than the defined MRL level, can cause many health effects, including allergic reactions, body's natural microflora removal, toxicity risks, antibiotic-resistant microorganisms, and reduced sensitivity to antibiotics, etc.
There are several methods for detecting antibiotics in raw milk, including HPLC, ELISA, gel diffusion, and clot testing, all of which are expensive, time-consuming, or inaccurate. Since milk is a nutritious food for microorganisms, they should be emptied and processed in the factory as soon as possible so that the least microbial growth happens in it, therefore milk antibiotics rapid test kit are used in laboratories for this purpose.
But since these kits are all imported, by localizing their production, it is possible to reduce the cost of testing and proper interaction with the manufacturer in order to achieve the defined MRL levels in national standards or to control local antibiotics used in the country and even start exporting them. On average, the applicant departments need 60 milk antibiotics test kits per day and intend to acquire the technical knowledge of producing these kits from skilled teams.

To purchase milk antibiotics rapid test kits (BALLYA brand), please contact us via following numbers:
Special No.:  +9821 42190          EXT: 160

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